GHRH Analog (Mod GRF 1-29)

CJC-1295 (NO DAC)

A modified fragment of growth hormone-releasing hormone with four amino acid substitutions for enhanced stability and short-acting, pulsatile GH release.

What Is CJC-1295 (No DAC)?

CJC-1295 without Drug Affinity Complex (No DAC), also known as Modified GRF (1-29), is a synthetic analog of the first 29 amino acids of growth hormone-releasing hormone. Four amino acid substitutions at positions 2, 8, 15, and 27 protect it from rapid enzymatic degradation, extending its functional half-life from under 10 minutes to approximately 30 minutes.

Unlike the DAC version which binds to albumin for extended circulation, the No DAC variant produces short, intense GH pulses that closely mimic the body's natural secretion pattern. This makes it the preferred research variant for preserving physiological GH rhythms.

Mechanism of Action

CJC-1295 (No DAC) acts on the same GHRH receptor pathway as endogenous GHRH, but with greater resistance to DPP-IV enzymatic cleavage. Key mechanisms include:

Binds GHRH receptors on anterior pituitary somatotrophs, triggering cAMP-mediated GH release
Produces acute, high-amplitude GH pulses lasting 2 – 3 hours per injection
Preserves natural negative feedback via somatostatin, preventing GH overproduction
Synergizes with GH-releasing peptides (GHRPs) which act on the ghrelin receptor
Elevates IGF-1 through downstream hepatic signaling

Research Applications

Growth hormone optimization and anti-aging research
Body composition studies (fat loss, lean mass preservation)
Sleep architecture and deep sleep enhancement
Injury recovery and connective tissue repair models
Synergistic GH protocols paired with GHRPs (ipamorelin, hexarelin)
IGF-1 elevation and downstream anabolic signaling

Dosing Protocol

CJC-1295 (No DAC) is administered via subcutaneous injection 2 – 3 times daily, typically before meals and at bedtime. The protocol below reflects commonly reported research dosing:

Protocol	Dose	Frequency	Notes
Standalone	100 mcg	2 – 3x daily	Before meals or at bedtime
With Ipamorelin	100 mcg each	2 – 3x daily	Combined injection, synergistic
Pre-bed pulse	100 – 200 mcg	Once nightly	Amplifies nocturnal GH surge

Inject subcutaneously on an empty stomach. Avoid eating for 20 – 30 minutes post-injection to prevent insulin-mediated GH blunting. Typical research cycles run 8 – 16 weeks.

Preparation & Storage

Reconstitution

Supplies: peptide vial, bacteriostatic water, insulin syringes (29–31 gauge), alcohol swabs.

Clean both vial tops with alcohol swabs and let dry
Draw 2 mL of bacteriostatic water into the syringe
Insert needle into peptide vial at an angle against the glass wall
Let water run slowly down the side – never squirt directly onto powder
Gently swirl until fully dissolved – never shake
Solution should be clear – discard if cloudy or contains particles

Concentration: 2 mg in 2 mL = 1,000 mcg/mL

Storage

Unreconstituted: Room temp or refrigerated, stable up to 24 months
Reconstituted: Refrigerate at 2–8°C (36–46°F), use within 4–6 weeks
Protect from direct sunlight and heat
Do not freeze reconstituted solution

Cycling

Typical research cycle: 8–12 weeks. Results generally reported within 2–4 weeks.