EDR Bioregulatory Peptide

Pinealon

Short bioregulatory peptide (Glu-Asp-Arg) for neuroprotection, pineal gland support, and sleep cycle regulation in research models.

◆ Evidence Level: Primarily Animal ResearchPinealon is a short peptide studied in cell culture and animal models for neuroprotective effects. No human clinical trials have been published. See references →

What Is Pinealon?

Pinealon is a synthetic tripeptide with the amino acid sequence Glu-Asp-Arg (EDR). It belongs to the Khavinson peptide bioregulator family, originally developed at the St. Petersburg Institute of Bioregulation and Gerontology. Pinealon was designed to target brain and central nervous system tissue, particularly the pineal gland.

As a bioregulatory peptide, Pinealon is theorized to interact with DNA at the gene level, potentially influencing the expression of proteins involved in neuroprotection and circadian rhythm regulation. Its small size (just three amino acids) allows it to cross the blood-brain barrier in research models.

Mechanism of Action

Gene Expression Regulation

Pinealon is believed to interact directly with DNA sequences, modulating the transcription of genes involved in neuronal survival, antioxidant defense, and melatonin synthesis. This epigenetic mechanism distinguishes it from traditional neuroprotective agents.

Pineal Gland Support

Research suggests Pinealon may help maintain pineal gland function by supporting melatonin production pathways. The pineal gland’s calcification and functional decline with age is a key area of interest for this peptide.

Antioxidant & Anti-Apoptotic Activity

In cell culture studies, Pinealon has demonstrated the ability to reduce reactive oxygen species (ROS) accumulation and inhibit apoptotic pathways in neuronal tissue, suggesting a direct cytoprotective role.

Research Applications

Neuroprotection – Investigated for reducing oxidative damage and apoptosis in CNS tissue models
Sleep & Circadian Regulation – Studied for supporting melatonin synthesis and normalizing sleep-wake cycles
Cognitive Function – Explored for potential improvements in memory and learning in aged animal models
Anti-Aging (Brain) – Researched as part of bioregulatory peptide protocols targeting age-related neurodegeneration
Pineal Gland Restoration – Investigated for counteracting age-related calcification and functional decline of the pineal gland

Dosing Protocols

Protocol	Dose	Frequency	Duration
Neuroprotection	5–10mg	1x daily	20–30 days
Sleep Support	5–10mg	1x daily (PM)	30 days
Cognitive Enhancement	10mg	1–2x daily	20–30 days

Bioregulatory peptides are typically administered in cycles of 10–30 days, followed by a rest period of equal length before repeating.

Preparation & Storage

Reconstitution

Supplies: peptide vial, bacteriostatic water, insulin syringes (29–31 gauge), alcohol swabs.

Clean both vial tops with alcohol swabs and let dry
Draw 2 mL of bacteriostatic water into the syringe
Insert needle into peptide vial at an angle against the glass wall
Let water run slowly down the side – never squirt directly onto powder
Gently swirl until fully dissolved – never shake
Solution should be clear – discard if cloudy or contains particles

Concentration: 10 mg in 2 mL = 5,000 mcg/mL

Storage

Unreconstituted: Room temp or refrigerated, stable up to 24 months
Reconstituted: Refrigerate at 2–8°C (36–46°F), use within 4–6 weeks
Protect from direct sunlight and heat
Do not freeze reconstituted solution

Cycling

Typical research cycle: 2–4 weeks. Results generally reported within 1–2 weeks.